Volume-activated Cl(-)-independent and Cl(-)-dependent K+ pathways in trout red blood cells.

GSH depletion, K-Cl cotransport, and regulatory volume decrease in high-K/high-GSH dog red blood cells.

Thiol reagents activate K-Cl cotransport (K-Cl COT), the Cl-dependent and Na-independent ouabain-resistant K flux, in red blood cells (RBCs) of several species, upon depletion of cellular glutathione (GSH). K-Cl COT is physiologically active in high potassium (HK), high GSH (HG) dog RBCs. In this unique model, we studied whether the same inverse relationship exists between GSH levels and K-Cl C...

NH4+ inhibits K-Cl Cotransport in Low K Sheep Red Blood Cells

Effect of changes in respiratory blood parameters on equine red blood cell K-Cl cotransporter.

K influx into equine red blood cells (RBCs) was measured using86Rb as a tracer for K under conditions designed to mimic the changes in respiratory blood parameters that occur in vivo during strenuous exercise. The effects on K influx of physiological changes in pH, cell volume, O2 tension ([Formula: see text]), CO2 tension ([Formula: see text]), and bicarbonate and lactate concentrations were d...

Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl- channels in human osteoblasts.

The whole cell recording mode of the patch-clamp technique was used to study the effect of hypotonic NaCl or isotonic high-KCl solution on membrane currents in a human osteoblast-like cell line, C1. Both hypotonic NaCl or isotonic high-KCl solution activated Cl- channels expressed in these cells as described previously. The reversal potential of the induced Cl- current is more negative when act...

Ionic events during the volume response of human peripheral blood lymphocytes to hypotonic media. I. Distinctions between volume- activated Cl- and K+ conductance pathways

Hypotonic dilution of human peripheral blood lymphocytes (PBL) induces large conductive permeabilities for K+ and Cl-, associated with the capacity of the cells to regulate their volumes. When rapid cation leakage is assured by the addition of the ionophore gramicidin, the behavior of the anion conductance pathway can be independently examined. Using this technique it is demonstrated that the v...